Laboratory Methods
Laboratory Methods
The Structural Biology program has transitioned from Common Fund support. For more information, please visit Program Snapshot page.
Please note that since the Structural Biology program is no longer supported by the Common Fund, the program website is being maintained as an archive and will not be updated on a regular basis.
Please note that since the Structural Biology program is no longer supported by the Common Fund, the program website is being maintained as an archive and will not be updated on a regular basis.
The Common Fund supported Structural Biology Program has funded the Joint Center for Innovative Membrane Protein Technologies-Complexes (JCIMPT-Complexes) and the Membrane Protein Expression Center (MPEC). These centers are research networks comprised of multiple integrated projects to develop new technologies and methods to enable the production of large amounts of homogeneous membrane proteins and determine protein structure.
Experimental Protocols
- Viral Stock generation
- Cell free Protein Production
- Membrane Prep
- Micro-Purification
- Large-Scale Protein Purification
- Preparation of Cholesterol in Detergent
- Measure Cell Surface Expression
- Microcoil NMR
- IMP Refolding for NMR Studies
Video Tutorial
Experimental Protocols
Techniques for membrane protein research
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A general protocol for the crystallization of membrane proteins for X-ray structural investigation
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Cell-free complements in vivo expression of E. coli membrane proteome
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A high throughput assay for membrane protein stabilization by ligands, buffers, detergents, lipids; and high quality membrane proteins in S. cerevisciae
