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Laboratory Methods

The Building Blocks, Biological Pathways and Networks program has transitioned from Common Fund support.  For more information, please visit Please note that since the Building Blocks, Biological Pathways and Networks program is no longer supported by the Common Fund, the program website is being maintained as an archive and will not be updated on a regular basis. 

BOEKE Protocols

  • Yeast high efficiency transformation - dSLAM (PDF) 
  • Yeast sporulation - dSLAM (PDF) 

COLE Protocols

  • Chemical synthesis of a novel coenzyme A-based affinity probe (PDF) 

COTTER Protocols

  • Quantification of lysine acetylation (PDF) 
  • N-terminal derivatization for de novo sequencing (PDF) 
  • Determination of ubiquitylation sites (PDF) 
  • Determination of SUMOylation sites (PDF) 

Frommer Protocols

MBIC Protocals

  • Preparing stock solutions of Malachite Green derivatives 

MELUH Protocols

  • Indirect Immunofluorescence for Yeast (PDF) 
  • DAPI Staining Yeast (PDF) 
  • Chromatin Immunoprecipitation (ChIP) for Yeast (PDF)

PANDEY Protocols

  • 18O - labeling trypsin digest protocol for relative quantitation by mass spectrometry 

RANDO Protocols

  • Mononucleosome Chromatin Immunoprecipitation for Yeast (PDF) 

NCDIR Protocols

Disruption of Yeast or Bacteria Cells

Subcellular Fractionation

Isolation of Protein Complexes

Mass Spectrometry

  • In-gel Digestion protocol 

Genomic Analyses

Protein Biochemistry

This page last reviewed on April 12, 2024