Program Resources

Liquid being distributed among small tubesLearn more about the resources being developed by the NIH Common Fund Glycoscience program:

GlyGen

GlyGen is a data integration and dissemination project that retrieves information from multiple international data sources. Click below to access tutorials on using GlyGen and other online GlyGen resources.

GLYCAM

  GLYCAM is a tool that facilitates computer modeling of carbohydrates.

CVLS4GAGs Beta

CVLS4GAGs Beta is a tool for modeling glycosaminoglycan interactions with proteins. The tool is available for use for investigators with an edu/org/gov affiliation.

 

More Resources Developed through the Glycoscience Program 

Resources Generated from the Glycoscience Program U01s 
Resource Description Publication(s) Availibility through Website and/or Company
Affinity reagents (arrays) Antibody-like probes in lampreys to glycans from different sources https://pubmed.ncbi.nlm.nih.gov/32052624/ NovAb, Inc. 
Quantitative O-glycomics and Amplification/Preparation of Cellular O-Glycans Technology to simulate synthesis of O-glycans in cell cultures https://pubmed.ncbi.nlm.nih.gov/32539345/ http://natglycan.com/
Glycam 3D structural modeling platform to predict glycan structures and their impact on polypeptide conformations of glycoproteins   https://www.glycam.org
IsoTag & IsoStamp Reagent for orthogonal metabolic labeling of glycans  

http://mass-spec-169.herokuapp.com/; 

Click Chemistry Tools: htttps://clickchemistrytools.com/isotag-kit/

GlycoMiR Integrated within GlycoEnzDB providing data on miRNA regulation of mRNA translation  https://pubmed.ncbi.nlm.nih.gov/34085516/; https://pubmed.ncbi.nlm.nih.gov/31585501/                  Integrated within http://www.virtualglycome.org/GlycoEnzDB
Affinity reagents (arrays) Harnesses sialic acid binding proteins found in human oral microflora as additional glycan binding affinity probes https://pubmed.ncbi.nlm.nih.gov/32820052/   
Luminex Bead Glycan Array Luminex beads and software to study glycan binding proteins https://pubmed.ncbi.nlm.nih.gov/34972929/; https://pubmed.ncbi.nlm.nih.gov/29343722/  
Probes N-acetyl muramic acid orthogonal metabolic probes https://pubmed.ncbi.nlm.nih.gov/31763799/;  https://pubmed.ncbi.nlm.nih.gov/29986130/  
Software for processing mass spectrometry glycomics and glycoproteomics data GlycReSoft is a tool for preprocessing and assigning glycoproteomics LC-MS data. GlycoDeNovo is a software tool for identification of glycans from ExD tandem mass spectra. GAGFinder and GAGrank are tools for annotating and ranking glycosaminoglycan saccharides from tandem mass spectra. https://pubmed.ncbi.nlm.nih.gov/33992776/; 
https://pubmed.ncbi.nlm.nih.gov/29790907/; 
https://pubmed.ncbi.nlm.nih.gov/29615495/; 
https://pubmed.ncbi.nlm.nih.gov/28786094/

https://www.bumc.bu.edu/msr/software/; 

GlycReSoft is available for download at: https://www.bumc.bu.edu/msr/glycresoft/;

Library of synthetic glycosaminoglycan standards funded by 3R21HL131554-02S1 distributed upon request: http://www.bumc.bu.edu/zaia/gag-synthetic-saccharides-available/;

GlycoDeNovo is available for download at: https://github.com/hongpengyu/GlycoDeNovo 

Glycan determination via suspension lectin arrays A near real-time technology using flow cytometry with bead-based glycan-specific reagents   www.lectenz.com
Glycopeptide Transition Wiki (GPTwiki) Provides optimized observed transitions for the quantification of peptides modified by glycans   https://gptwiki.glyomics.org/
GAG sequencing Automated capillary electrophoresis-­tandem mass spectrometry platform for the structural analysis of mixtures of glycosaminoglycan oligomers that are isolated in protein binding assays https://pubmed.ncbi.nlm.nih.gov/29501428/; https://pubmed.ncbi.nlm.nih.gov/30773923/  
Biosensors of GalNAc transferase activity Protein-based fluorescence sensors that traffic in the secretory pathway to monitor GalNAc-transferase activity in living cells. The sensors can either be “pan” or isozyme specific.    https://pubmed.ncbi.nlm.nih.gov/27632006/; https://pubmed.ncbi.nlm.nih.gov/25225288/  
T3Inh-1 Membrane-permeant, isozyme-selective, small molecule inhibitor of GalNAc-T3. Effective in cells and mice. Mechanism is direct and mixed-mode. https://pubmed.ncbi.nlm.nih.gov/28362263/  
Glycotuners (400 constructs) A suite of tools that enable small molecular regulation (Trimethoprim/Shield) of the expression of the O-GlcNAc transferase (OGT), O-GlcNAcase (OGT), Glutamine Fructose-6phophate aminotransferase 1, 2 (GFAT1,2), and UDP-N-Acetylglucosamine Pyrophosphorylase (UAP1/2).  https://pubmed.ncbi.nlm.nih.gov/34004049/  
UGAcS and UXPS Genetically encoded fluorescent indicator for UDP-GlcNAc, and the control indicator UXPS for UTP and UDP https://pubmed.ncbi.nlm.nih.gov/34729420/ http://www.addgene.org/Huiwang_Ai/
microbial Glycan Analysis Probes (mGAPs) Microbial glycan analysis probes (mGAPs) are based on soluble human lectins https://pubmed.ncbi.nlm.nih.gov/33497192/; https://pubmed.ncbi.nlm.nih.gov/34615357/; https://pubmed.ncbi.nlm.nih.gov/34145348/  
Photocrosslinking GlcNAc analog Method and reagents to identify cellular binding partners of glycosylated molecules https://pubmed.ncbi.nlm.nih.gov/34331854/; https://pubmed.ncbi.nlm.nih.gov/34288588/ https://www.utsouthwestern.edu/labs/kohler/reagents/photocrosslinking-gl...
Writing and erasing O-GlcNAc from target proteins in cells Fused nanobodies to O-GlcNAc transferase (OGT) or O-GlcNAcase (OGA) as proximity-directing agents to selectively “write” and “erase” O-GlcNAc from a target protein in cells https://pubmed.ncbi.nlm.nih.gov/33686291/; https://pubmed.ncbi.nlm.nih.gov/32119511/  
Tools, Clinical assays

1. Antibody array for analysis of N-glycans on IgG and IgG subtypes;

2. Immune cell N-glycan profiling after capture by antibody array;

3. N-glycan profiling of cultured cells;

4. Total N-glycan profiling of serum, plasma, urine, exosomes and other biofluids

1. https://pubmed.ncbi.nlm.nih.gov/35197973/;   https://pubmed.ncbi.nlm.nih.gov/31721442/

2. Manuscript in prepration;

3. https://pubmed.ncbi.nlm.nih.gov/31535553/

4.https://pubmed.ncbi.nlm.nih.gov/32809822/; https://pubmed.ncbi.nlm.nih.gov/34646811/

 
CVLS For GAGs A computational tool for understanding GAG recognition of proteins https://pubmed.ncbi.nlm.nih.gov/29328962/; https://pubmed.ncbi.nlm.nih.gov/28124818/; https://pubmed.ncbi.nlm.nih.gov/32346466/ https://cvls4gags.vcu.edu/wmol/
Glycan Libraries & Arrays  Chemical release of glycans from natural sources https://pubmed.ncbi.nlm.nih.gov/27135973/; https://pubmed.ncbi.nlm.nih.gov/29803109/; https://pubmed.ncbi.nlm.nih.gov/32277906/;
https://pubmed.ncbi.nlm.nih.gov/32539345/
NatGlycans
Chemical synthesis of glycosulfopeptides Scalable synthetic methodologies for GSP libraries; Development of methods for solid phase peptide synthesis  https://pubmed.ncbi.nlm.nih.gov/29638128/; https://pubmed.ncbi.nlm.nih.gov/29065342/; https://pubmed.ncbi.nlm.nih.gov/25824568/  
Chemical & chemoenzymatic synthesis of GAGs Methods for the rapid synthesis of protected building blocks used for synthesis of GAG libraries with defined sulfation patterns; Chemical methods for the automation of GAG synthesis; GAG Libraries (up to 18mers)  https://pubmed.ncbi.nlm.nih.gov/31553820/; https://pubmed.ncbi.nlm.nih.gov/29062430/; https://pubmed.ncbi.nlm.nih.gov/26574650/; https://pubmed.ncbi.nlm.nih.gov/28340300/  Glycan Therapeutics
Chemoenzymatic synthesis of glycosphingolipids Chemoenzymatic methods for the total synthesis of glycosphingolipids; Necessary enzymes and substrates for assembly of easy-to-use reagent kits; Kits to prepare various glycolipids via chemoenzymatic synthesis https://pubmed.ncbi.nlm.nih.gov/34152144/; https://pubmed.ncbi.nlm.nih.gov/33760402/ Integrated Micro-Chromatography Systems (IMCS) Inc.
Chemoenzymatic synthesis of polysaccharides Methods for the chemoenzymatic synthesis of several different bacterial oligosaccharides a; Prepared and optimized enzymes for these purposes   Integrated Micro-Chromatography Systems (IMCS) Inc. 
Automation of chemical synthesis Methods to synthesize the building blocks required to make O-glycans; First automated routes for synthesis of linear rhamnose-based polysaccharides https://pubmed.ncbi.nlm.nih.gov/30121415/;
https://pubmed.ncbi.nlm.nih.gov/27775116/;
https://pubmed.ncbi.nlm.nih.gov/31614269/;  
https://pubmed.ncbi.nlm.nih.gov/32432478/;
https://pubmed.ncbi.nlm.nih.gov/32293636/;
https://pubmed.ncbi.nlm.nih.gov/29336575/;
https://pubmed.ncbi.nlm.nih.gov/34463017/
 
Automation of chemical synthesis An HPLC based automated synthesis platform, and development of synthetic methods and polymer supports for solid-phase glycan synthesis https://pubmed.ncbi.nlm.nih.gov/27575052/;
https://pubmed.ncbi.nlm.nih.gov/27808325/;
https://pubmed.ncbi.nlm.nih.gov/29227649/;
https://pubmed.ncbi.nlm.nih.gov/29693690/;
https://pubmed.ncbi.nlm.nih.gov/29861508/;
https://pubmed.ncbi.nlm.nih.gov/30147293/;
https://pubmed.ncbi.nlm.nih.gov/30407673/;
https://pubmed.ncbi.nlm.nih.gov/31066275/;
https://pubmed.ncbi.nlm.nih.gov/31930269/;
https://pubmed.ncbi.nlm.nih.gov/32785304/;
 
Automation of chemoenzymatic synthesis Methods for automation of chemoenzymatic synthesis; Methods to prepare building blocks for use on his automated platform for chemoenzymatic synthesis of glycans; Commercialized a library of enzymes suitable for these purposes; Several glycans and glycan libraries have been synthesized and some have been used to prepare glycan arrays   https://pubmed.ncbi.nlm.nih.gov/30792508/;
https://pubmed.ncbi.nlm.nih.gov/29603480/;
https://pubmed.ncbi.nlm.nih.gov/30532014/;  
https://pubmed.ncbi.nlm.nih.gov/29096185/

Glyco Expression Technology (https://www.glycoexpresstech.com/);

Zymtronix Catalytic Systems, Inc (https://zymtronix.com/);

VIAMUNE, Inc (https://gra.org/company/84/ViaMune.html)

Automation of chemoenzymatic synthesis Glyco-enzymes (for synthetic purposes) developed and standard protocols for chemoenzymatic synthesis of O-glycans, Glycosphingolipids (GSLs) and Bacterial Polysaccharides (PSs); Machine driven approach for automatic synthesis of glycans and glycopeptides (using a peptide synthesizer) https://pubmed.ncbi.nlm.nih.gov/29732660/;
https://pubmed.ncbi.nlm.nih.gov/30375138/
Chemily Glycoscience sells the glycans, enzymes, and arrays created with them (http://www.chemilyglycoscience.com/); z BIOTECH, LLC sells arrays containing these libraries (https://www.zbiotech.com/)
Catalytic methods development  Bis-thiourea catalysts (that promote stereospecific reactions between a wide variety of complex nucleophiles and glycosyl phosphate donors) ; Methods for their highly selective and practical beta-mannosylation of alcohols, phenols, and thiols ; Progress toward site selective glycosylations of polyfunctional nucleophiles  through catalyst design https://pubmed.ncbi.nlm.nih.gov/30559190/;
https://pubmed.ncbi.nlm.nih.gov/28082586/;
https://pubmed.ncbi.nlm.nih.gov/32013410/;
https://pubmed.ncbi.nlm.nih.gov/32527078/
 
Catalytic methods development  Site-selective alkylation methods for both trans and cis -1,2-diols of O-glycosides; S-adamantyl group directed highly site-selective acylation of the C2-OH of various S glycosides; Mild way to prepare glycosyl chlorides and glycosyl bromides for cross-coupling glycosylation https://pubmed.ncbi.nlm.nih.gov/32431565/;
https://pubmed.ncbi.nlm.nih.gov/32026682/;
https://pubmed.ncbi.nlm.nih.gov/31739665/;
https://pubmed.ncbi.nlm.nih.gov/31066162/
 
Catalytic methods development  Stereoselective glycosylation reactions for the direct construction of beta-linked glycans using appropriately selected sulfonyl chloride promoters in the absence of directing groups; Construction of rare deoxy-sugar monosaccharides including a novel gold-catalyzed process for the cyclization of homopropargyl orthoformates; Automation of these new methods https://pubmed.ncbi.nlm.nih.gov/29572995/;
https://pubmed.ncbi.nlm.nih.gov/31021647/;
https://pubmed.ncbi.nlm.nih.gov/31305082/;
https://pubmed.ncbi.nlm.nih.gov/32281384/;
https://pubmed.ncbi.nlm.nih.gov/32293636/;
https://pubmed.ncbi.nlm.nih.gov/34463017/
 
Catalytic methods development  New catalytic methods utilizing a phenathroline catalyst or a visible-light-mediated copper catalyst for the synthesis of glycans https://pubmed.ncbi.nlm.nih.gov/30920099/;
https://pubmed.ncbi.nlm.nih.gov/34168901/;
https://pubmed.ncbi.nlm.nih.gov/29520326/
 
Catalytic methods development  A novel fluorescent multifunctional neoglycoside auxiliary (N-(2-ThioEthyl)-2-AminoBenzamide, (TEAB) synthesized as a versatile reagent for preparing thiol-functionalized glycans in high yield https://pubmed.ncbi.nlm.nih.gov/33761407/;
https://pubmed.ncbi.nlm.nih.gov/34264248/
 
Catalytic methods development  New catalytic methods for the synthesis of building blocks for glycan synthesis and for the conversion of glycan isomers (D to L)   https://pubmed.ncbi.nlm.nih.gov/34029464/;
https://pubmed.ncbi.nlm.nih.gov/32786781/;
https://pubmed.ncbi.nlm.nih.gov/29148749/;
https://pubmed.ncbi.nlm.nih.gov/30194299/
 
Synthetic methods development for carbohydrate synthesis New micro-wave assisted methods for the chemical synthesis of glycans https://pubmed.ncbi.nlm.nih.gov/34343001/;
https://pubmed.ncbi.nlm.nih.gov/33350830/;
https://pubmed.ncbi.nlm.nih.gov/32320231/
 
Synthetic methods development for carbohydrate synthesis Building block assembly and novel strategies for assembling glycosidic linkages; A simple method of synthesizing carbohydrates (performed at room temperature with commercial reagents without purification or drying)  https://pubmed.ncbi.nlm.nih.gov/34705439/;
https://pubmed.ncbi.nlm.nih.gov/33354969/;
https://pubmed.ncbi.nlm.nih.gov/32207615/  
 
Catalytic methods development  New catalytic methods for the synthesis of glycans  https://pubmed.ncbi.nlm.nih.gov/30439547/;
https://pubmed.ncbi.nlm.nih.gov/31396001/;
https://pubmed.ncbi.nlm.nih.gov/32431565/
 

 

 

      Websites

      The GECKO Project: CRISPR/Cas9-based molecular biology for validated human glycosyltransferase knock out cell lines

      Haab Lab: Software for analyzing glycan-array data, More information here and here

      Kohler Lab: Photocrosslinking sugar technology that can be used to identify glycan-dependent interactions

      Zaia Research Group: GAG synthetic saccharides

      National Center for Functional Glycomics: Lectin Reference Data 
      Wells and West Labs: Synthetic O-Glycan Standards
      Bertozzi Group: IsoTag Kit for Profiling Intact Glycopeptides and Isostamp v2.0 to analyze IsoTag data
      Li Lab: Info on synthetic useful glyco-enzymes and standard protocols for chemoenzymatic synthesis of O-glycans, glycosphingolipids, and bacterial polysaccharides

This page last reviewed on May 6, 2022