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Laboratory Methods

The Building Blocks, Biological Pathways and Networks program has transitioned from Common Fund support. For more information, please visit https://commonfund.nih.gov/bbpn/index. Please note that since the Building Blocks, Biological Pathways and Networks program is no longer supported by the Common Fund, the program website is being maintained as an archive and will not be updated on a regular basis.

BOEKE Protocols

Yeast high efficiency transformation - dSLAM (PDF)
Yeast sporulation - dSLAM (PDF)

COLE Protocols

Chemical synthesis of a novel coenzyme A-based affinity probe (PDF)

COTTER Protocols

Quantification of lysine acetylation (PDF)
N-terminal derivatization for de novo sequencing (PDF)
Determination of ubiquitylation sites (PDF)
Determination of SUMOylation sites (PDF)

Frommer Protocols

Protocol for monitoring intracellular metabolite levels

MBIC Protocals

Preparing stock solutions of Malachite Green derivatives

MELUH Protocols

Indirect Immunofluorescence for Yeast (PDF)
DAPI Staining Yeast (PDF)
Chromatin Immunoprecipitation (ChIP) for Yeast (PDF)

PANDEY Protocols

18O - labeling trypsin digest protocol for relative quantitation by mass spectrometry

RANDO Protocols

Mononucleosome Chromatin Immunoprecipitation for Yeast (PDF)

NCDIR Protocols

Disruption of Yeast or Bacteria Cells

Subcellular Fractionation

Isolation of Protein Complexes

Mass Spectrometry

In-gel Digestion protocol

Genomic Analyses

mRNA expression microarrays

Protein Biochemistry

This page last reviewed on November 4, 2019

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